We are creating a diagnostic platform that will address the key weaknesses in currently available in-vitro diagnostics and molecular diagnostic technologies. This platform, the Single Particle Interferometric Reflectance Imaging Sensor (SP-IRIS), is based on the detection of single nanoparticles and has demonstrated single virus and single molecule (both protein & nucleic acids) detection in whole blood and serum at clinically relevant levels.
The SP-IRIS platform has the advantage over other diagnostic technologies by allowing clinically relevant detection sensitivity without any complicated sample preparation or signal amplification. Even though other technologies have overcome sample preparation and amplification hurdles, the integration of these sample preparation solutions has made competitor technology more complicated, expensive, and slower. Finally, because SP-IRIS can identify both antibodies and antigens on the same test, a single panel can provide infection and immunological responses.
Over the next five years we will create a diagnostic platform that can be applied to a variety of different biological applications. Some of the applications we are currently working towards are a Viral Hemorrhagic Fever panel (Ebola, Lassa, Marburg) in collaboration with Boston University with support from NIH and an allergy panel with support from CIMIT.
The Single Particle Interferometric Reflectance Imaging Sensor (SP-IRIS) technology, from Nexgen Arrays, allows detection of biomolecules with single target (virus/protein/molecule) resolution. The SP-IRIS technology uses a substrate where tens of targets can be monitored in one test. The substrate is contained inside a cartridge that runs the test and can be scanned using the SP-IRIS imager. Possible applications include biosurveillance, pharmaceutical, diagnostics, and environmental monitoring.
The SP-IRIS imager is able to count individual nanoparticles (e.g. virus), as small as 60 nm, on the sensor surface using a capture probe, without amplification. For smaller particles, (e.g. genes, proteins) nanoparticle tags can be used to count single biomolecules.
Multiple targets on a single substrate
Up to 10s of targets can be detected
Direct detection from complex fluids
Blood, serum, and other biological fluids
Non-metallic nanoparticle detection (60 nm – 200 nm), Viruses, synthetic particles, and other nanoparticles can be accurately sized
Single-Particle Detection of Small Molecules
Proteins, Nucleic Acids, and toxins can be detected using metallic nanoparticles